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The Flower and Pre-fertilization Structures
- Flowers are morphological and embryological marvels and the sites of sexual reproduction.
- Stamen consists of two parts: the long slender stalk called the filament and the terminal bilobed structure called the anther.
- A typical angiosperm anther is bilobed and dithecous (two thecae per lobe).
- The anther is a four-sided structure consisting of four microsporangia located at the corners.
- Microsporangia develop further and become pollen sacs.
- The microsporangium wall has four layers: epidermis, endothecium, middle layers, and tapetum.
- The tapetum nourishes the developing pollen grains and possesses dense cytoplasm and generally more than one nucleus.
- Sporogenous tissue occupies the center of each microsporangium in young anthers.
- Microsporogenesis is the process of formation of microspores from a Pollen Mother Cell (PMC) through meiosis.
- Microspores are arranged in a cluster of four cells called the microspore tetrad.
Pollen Grain (Male Gametophyte)
- Pollen grains represent the male gametophytes.
- The two-layered wall of pollen consists of a hard outer exine and an inner intine.
- Exine is made of sporopollenin, one of the most resistant organic materials known.
- Germ pores are apertures in the exine where sporopollenin is absent.
- The intine is a thin, continuous layer made of cellulose and pectin.
- A mature pollen grain contains two cells: the vegetative cell (larger, abundant food reserve) and the generative cell (smaller, floats in cytoplasm).
- In over 60% of angiosperms, pollen is shed at the 2-celled stage.
- Pollen allergy (e.g., from Parthenium or carrot grass) causes chronic respiratory disorders.
- Pollen viability varies (e.g., 30 minutes in rice/wheat; months in Rosaceae).
The Pistil and Megasporogenesis
- The Gynoecium represents the female reproductive part.
- A pistil consists of the stigma (landing platform), style (elongated slender part), and ovary (basal bulged part).
- Megasporangium is the technical term for the ovule.
- The ovule is attached to the placenta by a stalk called the funicle.
- The junction where the body of the ovule fuses with the funicle is called the hilum.
- Integuments are protective envelopes that encircle the nucellus except at the micropyle.
- The chalaza represents the basal part of the ovule, opposite the micropyle.
- Megasporogenesis is the formation of megaspores from the Megaspore Mother Cell (MMC).
- Only one megaspore remains functional, while the other three degenerate (Monosporic development).
- A mature female gametophyte (embryo sac) is 7-celled and 8-nucleate.
- The egg apparatus consists of two synergids and one egg cell.
- Filiform apparatus in synergids guides the entry of the pollen tube.
Pollination
- Autogamy is pollination within the same flower.
- Cleistogamous flowers never open and ensure seed-set even without pollinators.
- Geitonogamy is functionally cross-pollination but genetically autogamy.
- Xenogamy brings genetically different types of pollen grains to the stigma.
- Anemophily (wind pollination) requires light, non-sticky pollen and well-exposed stamens.
- Hydrophily (water pollination) is rare, seen in Vallisneria and Hydrilla.
- Entomophily (insect pollination) involves large, colorful, fragrant, and nectar-rich flowers.
- Emasculation (removal of anthers) and bagging are techniques used in artificial hybridization.
Fertilization and Post-Fertilization
- Pollen-pistil interaction is a dynamic process involving pollen recognition followed by promotion or inhibition.
- Syngamy is the fusion of one male gamete with the egg cell to form a diploid zygote.
- Triple fusion is the fusion of the second male gamete with two polar nuclei to form the triploid Primary Endosperm Nucleus (PEN).
- Double fertilization (Syngamy + Triple Fusion) is a unique event in flowering plants.
- The PEN develops into the endosperm, which provides nutrition to the embryo.
- Embryogeny stages: Zygote → Proembryo → Globular → Heart-shaped → Mature embryo.
- Dicot embryo has two cotyledons; Monocot embryo has one (called scutellum).
- Albuminous seeds retain endosperm (e.g., wheat, maize, castor); Non-albuminous consume it (e.g., pea, groundnut).
- Perisperm is the persistent residual nucellus (e.g., black pepper, beet).
- Apomixis is a form of asexual reproduction that mimics sexual reproduction (seed formation without fertilization).
- Polyembryony is the occurrence of more than one embryo in a seed (common in citrus).
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